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hydrogen peroxide block solution  (Thermo Fisher)


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    Thermo Fisher hydrogen peroxide block solution
    Hydrogen Peroxide Block Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydrogen+peroxide+block/Hydrogen+peroxide%2C+35%25+w%2Fw+aq%2E+soln%2E%2C+stab/10__31797_slash_vetbio__1790287-90-24-32
    Average 94 stars, based on 1 article reviews
    hydrogen peroxide block solution - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Systemic and mammary Inflammation and mammary gland development of Holstein dairy cows around dry-off and calving.
    Article Snippet: The primary antibodies included monoclonal mouse anti-human Ki67 antibody (1:200 dilution, clone MIB-1, Agilent, Santa Clara, CA), polyclone rabbit anti-human CD3 antibody (1:75 dilution, Agilent), monoclonal rabbit anti-human CD20 antibody (1:75 dilution, ThermoFisher Scientific), monoclonal mouse anti-bovine CD172a (DH59B) antibody (1:75 dilution, Washington State University monoclonal antibody center). .. The staining procedures included quenching (Lab Vision Hydrogen Peroxide Block, ThermoFisher Scientific) for 10 min, blocking with Lab Vision Ultra V Block (ThermoFisher Scientific) for 5 min, primary antibody incubation for 1 h, incubation with UltraVision ONE HRP Polymer (Thermo Fisher Scientific) for 30 min, and color development with 3,3′-diaminobenzidine (Thermo Fisher Scientific) for 2 to 10 min. Each step was followed by 1 rinse with TBS. .. Slides were then rinsed with distilled water, counterstained with hematoxylin (Leica Biosystems, Buffalo Grove, IL), dehydrated, and mounted.

    Article Title: Investigation of the effects of silymarin and vitamin C on kidney damage and aquaporin-2 downregulation in lithium-induced nephrogenic diabetes insipidus in rats.
    Article Snippet: .. Deparaffinized tissues were passed through a graded alcohol series and boiled in Buffer solution at ph:6 in a microwave oven (750 W) for 7 + 5 min for antigen retrieval. the tissues which were kept at room temperature for 20 min to ensure cooling after being boiled were washed with PBs (Phosphate Buffered saline, P4417, sigma-aldrich, Usa) for 3 × 5 min and then were incubated with hydrogen peroxide block solution for 5 min to prevent endogenous peroxidase activity (hydrogen Peroxide Block, ta-125-hP, lab Vision corporation, Usa). the tissues, which had been washed again with PBs for 3 × 5 min, were then treated with the Ultra V Block (ta–125-UB, lab Vision corporation, Usa) solution for 5 min to prevent nonspecific background staining and were incubated for 60 min with primary antibody, aQP2 (anti-aQP2 antibody, sc sc-515770 santa cruz Biotechnology, inc. Oregon, Usa). it was diluted 1:200, at room temperature in a humid environment. after the tissues underwent the primary antibody treatment, they were washed with PBs for 3 × 5 min and then incubated with the secondary antibody (biotinylated Goat anti-Polyvalent (anti-mouse/rabbit igG), tP–125-BN, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment. .. Following the secondary antibody treatment, the tissues were washed with PBs for 3 × 5 min, incubated with streptavidin Peroxidase (ts–125-hR, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment, and transferred to PBs. then the 3-amino-9 ethylcarbazole (aec) substrate + aec chromogen (aec substrate, ta-015, and has, aec chromogen, ta-002-hac, lab Vision corporation, Usa) solution was dropped onto the tissues.

    Blocking Assay:

    Article Title: Systemic and mammary Inflammation and mammary gland development of Holstein dairy cows around dry-off and calving.
    Article Snippet: The primary antibodies included monoclonal mouse anti-human Ki67 antibody (1:200 dilution, clone MIB-1, Agilent, Santa Clara, CA), polyclone rabbit anti-human CD3 antibody (1:75 dilution, Agilent), monoclonal rabbit anti-human CD20 antibody (1:75 dilution, ThermoFisher Scientific), monoclonal mouse anti-bovine CD172a (DH59B) antibody (1:75 dilution, Washington State University monoclonal antibody center). .. The staining procedures included quenching (Lab Vision Hydrogen Peroxide Block, ThermoFisher Scientific) for 10 min, blocking with Lab Vision Ultra V Block (ThermoFisher Scientific) for 5 min, primary antibody incubation for 1 h, incubation with UltraVision ONE HRP Polymer (Thermo Fisher Scientific) for 30 min, and color development with 3,3′-diaminobenzidine (Thermo Fisher Scientific) for 2 to 10 min. Each step was followed by 1 rinse with TBS. .. Slides were then rinsed with distilled water, counterstained with hematoxylin (Leica Biosystems, Buffalo Grove, IL), dehydrated, and mounted.


    Article Title: Worse survival of hepatocellular cancer patients with membranous insulin receptor overexpression.
    Article Snippet: Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Fig. 4. .. Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Fig. 4. ..

    Article Title: Investigation of the effects of silymarin and vitamin C on kidney damage and aquaporin-2 downregulation in lithium-induced nephrogenic diabetes insipidus in rats.
    Article Snippet: .. Deparaffinized tissues were passed through a graded alcohol series and boiled in Buffer solution at ph:6 in a microwave oven (750 W) for 7 + 5 min for antigen retrieval. the tissues which were kept at room temperature for 20 min to ensure cooling after being boiled were washed with PBs (Phosphate Buffered saline, P4417, sigma-aldrich, Usa) for 3 × 5 min and then were incubated with hydrogen peroxide block solution for 5 min to prevent endogenous peroxidase activity (hydrogen Peroxide Block, ta-125-hP, lab Vision corporation, Usa). the tissues, which had been washed again with PBs for 3 × 5 min, were then treated with the Ultra V Block (ta–125-UB, lab Vision corporation, Usa) solution for 5 min to prevent nonspecific background staining and were incubated for 60 min with primary antibody, aQP2 (anti-aQP2 antibody, sc sc-515770 santa cruz Biotechnology, inc. Oregon, Usa). it was diluted 1:200, at room temperature in a humid environment. after the tissues underwent the primary antibody treatment, they were washed with PBs for 3 × 5 min and then incubated with the secondary antibody (biotinylated Goat anti-Polyvalent (anti-mouse/rabbit igG), tP–125-BN, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment. .. Following the secondary antibody treatment, the tissues were washed with PBs for 3 × 5 min, incubated with streptavidin Peroxidase (ts–125-hR, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment, and transferred to PBs. then the 3-amino-9 ethylcarbazole (aec) substrate + aec chromogen (aec substrate, ta-015, and has, aec chromogen, ta-002-hac, lab Vision corporation, Usa) solution was dropped onto the tissues.


    Article Title: Worse survival of hepatocellular cancer patients with membranous insulin receptor overexpression
    Article Snippet: .. Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Scientific) for 15 min, washed with TBS and then blocked with Ultra V Block (Thermo Fisher Scientific) for 5 min. .. The ImmPRESS reagent peroxidase universal anti-mouse/rabbit IgG – MP-7500 and the ImmPact NovaRed peroxidase substrate SK-4805 Kit (Vector Laboratories, Burlingame, CA, USA, respectively) were used for the visualization of immunoreactions.

    Article Title: Neuroprotective Effects of Cilomilast and Chlorogenic Acid Against Scopolamine-Induced Memory Deficits via Modulation of the cAMP/PKA–CREB–BDNF Pathway
    Article Snippet: .. The activity of endogenous peroxidase was blocked by the addition of a hydrogen peroxide block (Thermo Scientific, Waltham, MA, USA). ..

    Article Title: Cerium oxide nanoparticles attenuate hepatic failure via blocking TGF-β/Smads and upregulating Nrf2/HO-1 signaling pathways in liver fibrosis rat model
    Article Snippet: .. To inhibit the activity of endogenous peroxidase, Hydrogen Peroxide Block (Thermo Scientific, USA) was incorporated. ..

    Incubation:

    Article Title: Systemic and mammary Inflammation and mammary gland development of Holstein dairy cows around dry-off and calving.
    Article Snippet: The primary antibodies included monoclonal mouse anti-human Ki67 antibody (1:200 dilution, clone MIB-1, Agilent, Santa Clara, CA), polyclone rabbit anti-human CD3 antibody (1:75 dilution, Agilent), monoclonal rabbit anti-human CD20 antibody (1:75 dilution, ThermoFisher Scientific), monoclonal mouse anti-bovine CD172a (DH59B) antibody (1:75 dilution, Washington State University monoclonal antibody center). .. The staining procedures included quenching (Lab Vision Hydrogen Peroxide Block, ThermoFisher Scientific) for 10 min, blocking with Lab Vision Ultra V Block (ThermoFisher Scientific) for 5 min, primary antibody incubation for 1 h, incubation with UltraVision ONE HRP Polymer (Thermo Fisher Scientific) for 30 min, and color development with 3,3′-diaminobenzidine (Thermo Fisher Scientific) for 2 to 10 min. Each step was followed by 1 rinse with TBS. .. Slides were then rinsed with distilled water, counterstained with hematoxylin (Leica Biosystems, Buffalo Grove, IL), dehydrated, and mounted.

    Article Title: Investigation of the effects of silymarin and vitamin C on kidney damage and aquaporin-2 downregulation in lithium-induced nephrogenic diabetes insipidus in rats.
    Article Snippet: .. Deparaffinized tissues were passed through a graded alcohol series and boiled in Buffer solution at ph:6 in a microwave oven (750 W) for 7 + 5 min for antigen retrieval. the tissues which were kept at room temperature for 20 min to ensure cooling after being boiled were washed with PBs (Phosphate Buffered saline, P4417, sigma-aldrich, Usa) for 3 × 5 min and then were incubated with hydrogen peroxide block solution for 5 min to prevent endogenous peroxidase activity (hydrogen Peroxide Block, ta-125-hP, lab Vision corporation, Usa). the tissues, which had been washed again with PBs for 3 × 5 min, were then treated with the Ultra V Block (ta–125-UB, lab Vision corporation, Usa) solution for 5 min to prevent nonspecific background staining and were incubated for 60 min with primary antibody, aQP2 (anti-aQP2 antibody, sc sc-515770 santa cruz Biotechnology, inc. Oregon, Usa). it was diluted 1:200, at room temperature in a humid environment. after the tissues underwent the primary antibody treatment, they were washed with PBs for 3 × 5 min and then incubated with the secondary antibody (biotinylated Goat anti-Polyvalent (anti-mouse/rabbit igG), tP–125-BN, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment. .. Following the secondary antibody treatment, the tissues were washed with PBs for 3 × 5 min, incubated with streptavidin Peroxidase (ts–125-hR, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment, and transferred to PBs. then the 3-amino-9 ethylcarbazole (aec) substrate + aec chromogen (aec substrate, ta-015, and has, aec chromogen, ta-002-hac, lab Vision corporation, Usa) solution was dropped onto the tissues.

    Polymer:

    Article Title: Systemic and mammary Inflammation and mammary gland development of Holstein dairy cows around dry-off and calving.
    Article Snippet: The primary antibodies included monoclonal mouse anti-human Ki67 antibody (1:200 dilution, clone MIB-1, Agilent, Santa Clara, CA), polyclone rabbit anti-human CD3 antibody (1:75 dilution, Agilent), monoclonal rabbit anti-human CD20 antibody (1:75 dilution, ThermoFisher Scientific), monoclonal mouse anti-bovine CD172a (DH59B) antibody (1:75 dilution, Washington State University monoclonal antibody center). .. The staining procedures included quenching (Lab Vision Hydrogen Peroxide Block, ThermoFisher Scientific) for 10 min, blocking with Lab Vision Ultra V Block (ThermoFisher Scientific) for 5 min, primary antibody incubation for 1 h, incubation with UltraVision ONE HRP Polymer (Thermo Fisher Scientific) for 30 min, and color development with 3,3′-diaminobenzidine (Thermo Fisher Scientific) for 2 to 10 min. Each step was followed by 1 rinse with TBS. .. Slides were then rinsed with distilled water, counterstained with hematoxylin (Leica Biosystems, Buffalo Grove, IL), dehydrated, and mounted.

    Activity Assay:


    Article Title: Investigation of the effects of silymarin and vitamin C on kidney damage and aquaporin-2 downregulation in lithium-induced nephrogenic diabetes insipidus in rats.
    Article Snippet: .. Deparaffinized tissues were passed through a graded alcohol series and boiled in Buffer solution at ph:6 in a microwave oven (750 W) for 7 + 5 min for antigen retrieval. the tissues which were kept at room temperature for 20 min to ensure cooling after being boiled were washed with PBs (Phosphate Buffered saline, P4417, sigma-aldrich, Usa) for 3 × 5 min and then were incubated with hydrogen peroxide block solution for 5 min to prevent endogenous peroxidase activity (hydrogen Peroxide Block, ta-125-hP, lab Vision corporation, Usa). the tissues, which had been washed again with PBs for 3 × 5 min, were then treated with the Ultra V Block (ta–125-UB, lab Vision corporation, Usa) solution for 5 min to prevent nonspecific background staining and were incubated for 60 min with primary antibody, aQP2 (anti-aQP2 antibody, sc sc-515770 santa cruz Biotechnology, inc. Oregon, Usa). it was diluted 1:200, at room temperature in a humid environment. after the tissues underwent the primary antibody treatment, they were washed with PBs for 3 × 5 min and then incubated with the secondary antibody (biotinylated Goat anti-Polyvalent (anti-mouse/rabbit igG), tP–125-BN, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment. .. Following the secondary antibody treatment, the tissues were washed with PBs for 3 × 5 min, incubated with streptavidin Peroxidase (ts–125-hR, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment, and transferred to PBs. then the 3-amino-9 ethylcarbazole (aec) substrate + aec chromogen (aec substrate, ta-015, and has, aec chromogen, ta-002-hac, lab Vision corporation, Usa) solution was dropped onto the tissues.


    Article Title: Neuroprotective Effects of Cilomilast and Chlorogenic Acid Against Scopolamine-Induced Memory Deficits via Modulation of the cAMP/PKA–CREB–BDNF Pathway
    Article Snippet: .. The activity of endogenous peroxidase was blocked by the addition of a hydrogen peroxide block (Thermo Scientific, Waltham, MA, USA). ..

    Article Title: Cerium oxide nanoparticles attenuate hepatic failure via blocking TGF-β/Smads and upregulating Nrf2/HO-1 signaling pathways in liver fibrosis rat model
    Article Snippet: .. To inhibit the activity of endogenous peroxidase, Hydrogen Peroxide Block (Thermo Scientific, USA) was incorporated. ..

    Immunostaining:

    Article Title: Worse survival of hepatocellular cancer patients with membranous insulin receptor overexpression.
    Article Snippet: Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Fig. 4. .. Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Fig. 4. ..

    Article Title: Worse survival of hepatocellular cancer patients with membranous insulin receptor overexpression
    Article Snippet: .. Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Scientific) for 15 min, washed with TBS and then blocked with Ultra V Block (Thermo Fisher Scientific) for 5 min. .. The ImmPRESS reagent peroxidase universal anti-mouse/rabbit IgG – MP-7500 and the ImmPact NovaRed peroxidase substrate SK-4805 Kit (Vector Laboratories, Burlingame, CA, USA, respectively) were used for the visualization of immunoreactions.

    Saline:

    Article Title: Worse survival of hepatocellular cancer patients with membranous insulin receptor overexpression.
    Article Snippet: Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Fig. 4. .. Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Fig. 4. ..

    Article Title: Investigation of the effects of silymarin and vitamin C on kidney damage and aquaporin-2 downregulation in lithium-induced nephrogenic diabetes insipidus in rats.
    Article Snippet: .. Deparaffinized tissues were passed through a graded alcohol series and boiled in Buffer solution at ph:6 in a microwave oven (750 W) for 7 + 5 min for antigen retrieval. the tissues which were kept at room temperature for 20 min to ensure cooling after being boiled were washed with PBs (Phosphate Buffered saline, P4417, sigma-aldrich, Usa) for 3 × 5 min and then were incubated with hydrogen peroxide block solution for 5 min to prevent endogenous peroxidase activity (hydrogen Peroxide Block, ta-125-hP, lab Vision corporation, Usa). the tissues, which had been washed again with PBs for 3 × 5 min, were then treated with the Ultra V Block (ta–125-UB, lab Vision corporation, Usa) solution for 5 min to prevent nonspecific background staining and were incubated for 60 min with primary antibody, aQP2 (anti-aQP2 antibody, sc sc-515770 santa cruz Biotechnology, inc. Oregon, Usa). it was diluted 1:200, at room temperature in a humid environment. after the tissues underwent the primary antibody treatment, they were washed with PBs for 3 × 5 min and then incubated with the secondary antibody (biotinylated Goat anti-Polyvalent (anti-mouse/rabbit igG), tP–125-BN, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment. .. Following the secondary antibody treatment, the tissues were washed with PBs for 3 × 5 min, incubated with streptavidin Peroxidase (ts–125-hR, lab Vision corporation, Usa) for 30 min at room temperature in a humid environment, and transferred to PBs. then the 3-amino-9 ethylcarbazole (aec) substrate + aec chromogen (aec substrate, ta-015, and has, aec chromogen, ta-002-hac, lab Vision corporation, Usa) solution was dropped onto the tissues.

    Article Title: Worse survival of hepatocellular cancer patients with membranous insulin receptor overexpression
    Article Snippet: .. Identical immunostaining protocols were performed for both immunostaining reactions: First all sections were deparaffinized and afterwards boiled in EDTA buffer (pH 9.0; 1 min; 125 °C), then washed with Tris-buffered saline (TBS) and then treated with hydrogen peroxide block (Thermo Fisher Scientific) for 15 min, washed with TBS and then blocked with Ultra V Block (Thermo Fisher Scientific) for 5 min. .. The ImmPRESS reagent peroxidase universal anti-mouse/rabbit IgG – MP-7500 and the ImmPact NovaRed peroxidase substrate SK-4805 Kit (Vector Laboratories, Burlingame, CA, USA, respectively) were used for the visualization of immunoreactions.



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